COLORIMETRIC DETERMINATION OF UREA

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Colorimetric Determination of Urea by Reginald M. Archibald

The use of a-isonitrosopropiophenone, CS& *CO * CNOH. CH3, as a reagent for the calorimetric determination of urea has been mentioned in a previous communication (1). The present report outlines a simple method for the calorimetric determination of urea in blood filtrates and urine by means of this reagent and compares the values obtained by this method with those given by the urease ((2), (3) ...

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The Direct Colorimetric Determination of Urea in Blood and Urine

Methods for the determination of urea in biological fluids have passed through an interesting cycle (cf. Peters and Van Slyke (3) pp. 539-544). The earliest procedures were strictly chemical ones, involving precipitation of the urea as an insoluble mercury complex or decomposition of the urea by means of heat or hypobromite. The use of the highly specific enzyme urease occurring in the jack and...

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Colorimetric Determination of Urea by Reginald M. Archibald

The use of a-isonitrosopropiophenone, CS& *CO * CNOH. CH3, as a reagent for the calorimetric determination of urea has been mentioned in a previous communication (1). The present report outlines a simple method for the calorimetric determination of urea in blood filtrates and urine by means of this reagent and compares the values obtained by this method with those given by the urease ((2), (3) ...

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A sensitive method for the colorimetric determination of urea.

A method is described for the direct colorimetric determination of urea in biological fluids. The method depends on the reaction (first described by Wheatley, 1948) between urea, diacetylmonoxime, and phenylanthranilic acid in the presence of controlled amounts of oxidant; chloride ions are included to sensitize the reaction; manganous ions stabilize the resultant colour; and phosphate enables ...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1945

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)51085-1